Biofilm Origins, Oecology, and Metabolism
Exploring the extraordinary metabolic versatility of the microbes in and around us
My research program addresses two grand challenges in science: antimicrobial resistance and quantifying global biogeochemical cycling. In the first, I am interested in how diversity and metabolic changes in biofilms contribute to the alarming and expanding problem of antibiotic resistance. In the second, I study anaerobic metabolisms associated with biogeochemical cycles as a biotechnological and ecological source of innovation. Traditionally these disciplines don’t intersect. I would argue, however, that microbial ecology and evolution mediated by metabolic feedback lie at the core of each. Metabolic byproducts can open up new niches that influence eco-evolutionary dynamics and vice versa. Insights into diversification, resilience, resource competition, and cooperation in one system (environment) can inform another (host). Furthermore, the practical component uniting my research program is the study of ecology and evolution in anaerobic biofilms, particularly those that experience limiting resources (e.g. mucosal surfaces or natural environments). In addition, I am keenly interested in developing novel engineered devices and bioinformatic tools to address key scientific questions.
Scribner MR, Santos-Lopez A, Marshall CW, Deitrick C, Cooper VS. Parallel evolution of tobramycin resistance across species and environments. 2019. bioRxiv. https://doi.org/10.1101/758979.
Santos-Lopez* A, Marshall* CW, Scribner MR, Snyder DJ, Cooper VS. Evolutionary Pathways to Antibiotic Resistance are Dependent Upon Environmental Structure and Bacterial Lifestyle. 2019. eLife. 8:e47612.
Lopez S, Martin JM, Johnson M, Kurs-Lasky Horne WT, Marshall CW, Cooper VS, Williams JV, Shaikh N. A method of processing nasopharyngeal swabs to enable multiple testing. 2019. Pediatric Research. https://doi.org/10.1038/s41390-019-0498-1.
Payne RB, Ghosh U, May HD, Marshall CW, Sowers KR. A Pilot-Scale Field Study: In Situ Treatment of PCB-Impacted Sediments with Bioamended Activated Carbon. 2019. Environmental Science and Technology. DOI: 10.1021/acs.est.8b05019
Gloag* ES, Marshall* CW, Snyder DJ, Lewin GR, Harris JS, Chaney SB, Whiteley M, Cooper VS, Wozniak DJ. The Pseudomonas aeruginosa Wsp pathway undergoes positive evolutionary selection during chronic infection. 2019. mBio. 10 (4), e01698-19.
Dunlap DG, Marshall CW, Fitch A, Rapport SF, Cooper VS, McVerry B, Morris A, GD Kitsios. Bacterial DNA next generation sequencing can detect culprit pathogens missed by cultures and impact antibiotic management decisions in severe bacterial pneumonia. 2018. American Journal of Case Reports. 19, 1405-1409.
Mustapha M, Li B, Pacey M, Mettus R, McElheny C, Marshall CW, Ernst R, Cooper V, Doi Y. Phylogenomics of Colistin-Susceptible and Resistant, Extensively Drug-Resistant Acinetobacter baumannii. 2018. Journal of Antimicrobial Chemotherapy. 73 (11), 2952-2959.
Turner C, Marshall CW, Cooper V. Parallel genetic adaptation across environments differing in mode of growth or resource availability. 2018. Evolution Letters. doi.org/10.1002/evl3.75.
Melvin J, Gaston J, Phillips S, Springer M, Marshall CW, Shanks R, Bomberger J. Pseudomonas aeruginosa Contact-Dependent Growth Inhibition Plays a Dual Role in Host-Pathogen Interactions. 2017. mSphere. 2 (6). e00336-17.
Marshall CW, DE Ross, KM Handley, PB Weisenhorn, CS Henry, JA Gilbert, HD May, RS Norman. Metabolic Reconstruction and Modeling Microbial Electrosynthesis. 2017. Scientific Reports. 10.1038/s41598-017-08877-z (SREP-17-14408).
Ross DE, Marshall CW, May HD, Norman RS. Metagenome-Assembled Genome Sequences of Acetobacterium sp. Strain MES1 and Desulfovibrio sp. Strain MES5 from a Cathode-Associated Acetogenic Microbial Community. 2017. Genome Announcements. 5 (36), e00938-17.
Payne RB, Ghosh U, May HD, Marshall CW, Sowers KR. Mesocosm Studies on the Efficacy of Bioamended Activated Carbon for Treating PCB-Impacted Sediment. 2017. Environmental Science and Technology. doi: 10.1021/acs.est.7b01935
Wang H, Marshall CW, Cheng M, Xu H, Li H, Yang XR, Zheng T. Changes in land use driven urbanization impact nitrogen cycling and the microbial community composition in soils. 2017. Scientific Reports. 7, 44049.
Yang XR, Weng BS, Li H, Marshall CW, Li H, Chen YS, Yu S, Zhu GB, Zhu YG. An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China. Journal of Soils and Sediments. 2017. doi:10.1007/s11368-017-1728-y.
Zhou GW, Yang XR, Marshall CW, Li H, Zheng BX, Yan Y, Su JQ, Zhu YG. Biochar addition increases the rates of dissimilatory iron reduction and methanogenesis in ferrihydrite enrichments. Frontiers in Microbiology. 2017. 8: 589.
Zhou GW, Yang XR, Li H, Marshall CW, Zheng BX, Yan Y, Su JQ, Zhu YG. Electron Shuttles Enhance Anaerobic Ammonium Oxidation Coupled to Iron (III) Reduction. Environmental Science and Technology. 2016. 50 (17), 9298-9307.
Ross DE, CW Marshall, HD May, RS Norman. Comparative Genomic Analysis of Sulfurospirillum cavolei MES Reconstructed from the Metagenome of an Electrosynthetic Microbiome. PLoS One. 2016. 11(3). e0151214.
Burgsdorf I, BM Slaby, KM Handley, M Haber, J Blom, CW Marshall, JA Gilbert, U Hentschel, L Steindler. Lifestyle Evolution in Cyanobacterial Symbionts of Sponges. mBio. 2015. 6 (3): e00391-15.
Ross DE, CW Marshall, HD May, RS Norman. Draft Genome Sequence of Sulfurospirillum sp. Strain MES, Reconstructed from the Metagenome of a Microbial Electrosynthesis System. Genome Announcements. 2015. 3(1): e01336-14.
LaBelle EV, CW Marshall, JA Gilbert, HD May. Influence of Acidic pH on Hydrogen and Acetate Production by an Electrosynthetic Microbiome. PLoS One. 2014. 9(10): e109935.
Marshall CW, DR Ross, EB Fichot, RS Norman, HD May. Long-term Operation of Microbial Electrosynthesis Systems Improves Acetate Production by Autotrophic Microbiomes. Environmental Science and Technology. 2013. 47(11): 6023-6029.
Marshall CW, EV LaBelle, HD May. Production of Fuels and Chemicals from Waste by Microbiomes. Current Opinions in Biotechnology. 2013. 24(3): 391-397.
Marshall CW, DR Ross, EB Fichot, RS Norman, HD May. Electrosynthesis of Commodity Chemicals by an Autotrophic Microbial Community. Applied and Environmental Microbiology. 2012. 78(23): 8412-8420.
Marshall, Christopher W and Harold D May. Electrochemical evidence of direct electrode reduction by a thermophilic Gram-positive bacterium, Thermincola ferriacetica. Energy & Environmental Science. 2009, 2: 699-705.
Mathis, BJ, CW Marshall, CE Milliken, RS Makkar, SE Creager, HD May. Electricity generation by thermophilic microorganisms from marine sediment. Applied Microbiology and Biotechnology. 2008. 78(1): 147-155.
CW Marshall, EV LaBelle, HD May. Microbial Electrosynthetic Cells. 2014. Patent Publication Number WO 2014⁄043690 A1.
KR Sowers, HD May, CL Chun, CW Marshall. Thermophilic Methanogenic Consortium for Conversion of Cellulosic Biomass to Bioenergy. 2011. Patent Publication Number US20110306089.
(Past and Present)
Experimental Microbiology. Marquette University. Undergraduate. Fall 2019. BIO 4802.
Comprehensive Microbiology. University of Pittsburgh. Graduate level. Spring 2018 & Spring 2019. 2184 MSMI 1100.
Pseudomonas and Friends Journal Club. University of Pittsburgh.
Applied and Environmental Microbiology. Duquesne University. Graduate and Undergraduate level. Spring 2017. ENVI 497⁄597 BIO 432W/532.
The Microbiome and Health. Undergraduate level. Univeristy of Chicago. Spring 2015. BIOS 15123.
General Microbiology. Graduate and Undergraduate level. College of Charleston. 2009 – 2011. Bio 310 / 320.
Environmental Microbiology. Graduate level. MUSC. Spring 2010 - 2012. MBIM-772.